Defending Gold medal champion Serena Williams has been bundled out of the Rio Olympics by Ukrainian Elina Svitolina.
Williams lost her third round match 6-4, 6-3, after earlier in the week losing the first round in the doubles with sister Venus.
Meanwhile, Australian Sam Stosur is also out after a straight sets loss to Australian Open champion Angelique Kerber.
The world No.2 advanced to the fourth round with a 6-0, 7-5 win over the Aussie to end her medal hopes in the singles compeition a clinical performance.
Kerber will face Great Britain's Johanna Konta in the quarterfinals.
However, she is still a chance to win a medal for her country in doubles, with Stosur and John Peers set to take on Indian pair Sania Mirza and Rohan Bopanna in the first round of the mixed doubles.